Seminal Fluid, Acid Phosphatase Test (6 tests)

The Standard in Forensic Semen Detection

The BLUESTAR® Identi-PSA® test is a rapid, qualitative immunochromatographic assay for the detection of Prostate-Specific Antigen (PSA). It is specifically designed for forensic medicine use, allowing for its application in the lab, directly at the crime scene, or on the victim of a suspected sexual assault.

The Principle of the Test: PSA Detection

PSA is a glycoprotein synthesized by the prostate gland and secreted in high concentrations into seminal fluid. It has become the gold standard marker for detecting sexual assault, as it is also present in the semen of men who have undergone a vasectomy.

Key Forensic Advantages

• High Sensitivity: Detects PSA levels as low as 4 ng/ml.
• Fast Results: Provides a clear, legible result in just 10 minutes.
• Proven Reliability: Both intra- and inter-assay reproducibility studies show 100% correct identification of positive and negative results.
• High Specificity: The test uses monoclonal antibodies that do not cross-react with blood or other fluids, making it ideal for forensic use.
• Simple and Practical: Its ease of use is highly valued compared to the complex traditional ELISA method.

Simple Procedure (Key Steps)

1. COLLECT: Collect the sample (from clothing or body) with the included sterile swab. If the stain is dry, moisten the swab with the solution from the extraction vial.
2. MIX: Place the swab into the extraction vial, shake vigorously for 10 seconds, and squeeze the swab against the sides of the vial.
3. APPLY: Break the tip of the collection vial and dispense 4 drops into the sample well of the test cassette.

Interpreting the Results (at 10 minutes)

• Positive: TWO burgundy-colored lines appear (one in the Control "C" zone and one in the Test "T" zone). A faint "T" line is also considered a positive result.
• Negative: Only ONE line appears in the Control "C" zone.
• Invalid: No line appears in the Control "C" zone.

Kit Contents

Each kit contains all components necessary for 6 tests:

• 6 x BLUESTAR® Identi-PSA® test cassettes in protective pouches.
• 6 x Extraction vials (with 1 ml of buffer solution).
• 6 x Sterile swabs.
• 1 x Instructions for use.

Performance and Storage

• Accuracy: Has been evaluated (Hochmeister M., et al., 1997) as a test perfectly suited for forensic medicine due to its sensitivity, specificity, and simplicity compared to the ELISA method.
• Hook Effect: Samples with very high PSA concentrations (up to 10 µg/ml) yield positive results. However, if a negative result is obtained from a pure semen sample, it is recommended to re-test with a 1/100 or 1/1000 dilution.
• Storage: Store the kit at room temperature, between 2°C and 30°C. DO NOT FREEZE.
• Other: Also available in a 24-test pack.

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